Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
235 results
Cited 1 times since 2024 (3.1 per year) source: EuropePMC
Journal of the American Heart Association, Volume 13, Issue 3, 23 4 2024, Pages e033109 Myeloid PHD2 Conditional Knockout Improves Intraplaque Angiogenesis and Vascular Remodeling in a Murine Model of Venous Bypass Grafting. Sluiter TJ, Tillie RJHA, de Jong A, de Bruijn JBG, Peters HAB, van de Leijgraaf R, Halawani R, Westmaas M, Starink LIW, Quax PHA, Sluimer JC, de Vries MR
Background: Intraplaque angiogenesis occurs in response to atherosclerotic plaque hypoxia, which is driven mainly by highly metabolically active macrophages. Improving plaque oxygenation by increasing macrophage hypoxic signaling, thus stimulating intraplaque angiogenesis, could restore cellular function and neovessel maturation, and decrease plaque formation. Prolyl hydroxylases (PHDs) regulate cellular responses to hypoxia. We therefore aimed to elucidate the role of myeloid PHD2, the dominant... Abstract
International journal of molecular sciences, Volume 24, Issue 18, 7 1 2023, Pages 13822 Exploring the Effects of Human Bone Marrow-Derived Mononuclear Cells on Angiogenesis In Vitro. Peeters JAHM, Peters HAB, Videler AJ, Hamming JF, Schepers A, Quax PHA
Cell therapies involving the administration of bone marrow-derived mononuclear cells (BM-MNCs) for patients with chronic limb-threatening ischemia (CLTI) have shown promise; however, their overall effectiveness lacks evidence, and the exact mechanism of action remains unclear. In this study, we examined the angiogenic effects of well-controlled human bone marrow cell isolates on endothelial cells. The responses of endothelial cell proliferation, migration, tube formation, and aortic ring sprouti... Abstract
Frontiers in cardiovascular medicine, Volume 9, 16 3 2022, Pages 1094094 Editorial: Inflammation and immunomodulation in cardiovascular remodeling. Ewing M, Karper JC, de Vries MR, Quax PHA
Cited 1 times since 2022 (0.7 per year) source: EuropePMC
International journal of molecular sciences, Volume 23, Issue 21, 7 1 2022, Pages 13662 Phosphorylcholine Monoclonal Antibody Therapy Decreases Intraplaque Angiogenesis and Intraplaque Hemorrhage in Murine Vein Grafts. Baganha F, Sluiter TJ, de Jong RCM, van Alst LA, Peters HAB, Jukema JW, Delibegovic M, Pettersson K, Quax PHA, de Vries MR
Phosphorylcholine (PC) is one of the main oxLDL epitopes playing a central role in atherosclerosis, due to its atherogenic and proinflammatory effects. PC can be cleared by natural IgM antibodies and low levels of these antibodies have been associated with human vein graft (VG) failure. Although PC antibodies are recognized for their anti-inflammatory properties, their effect on intraplaque angiogenesis (IPA) and intraplaque hemorrhage (IPH)-interdependent processes contributing to plaque ruptur... Abstract
International journal of molecular sciences, Volume 23, Issue 21, 31 5 2022, Pages 13298 Visualization of Murine Vascular Remodeling and Blood Flow Dynamics by Ultra-High-Frequency Ultrasound Imaging. Sier VQ, de Jong A, Quax PHA, de Vries MR
Vein grafts (VGs) are used to bypass atherosclerotic obstructions and arteriovenous fistulas (AVFs) as vascular access for hemodialysis. Vascular remodeling governs post-interventional arterialization, but may also induce VG and AVF failure. Although the endpoint characteristics of vascular remodeling are known, the in vivo process and the role of blood flow dynamics has not been fully studied. Therefore, here we non-invasively quantify vascular remodeling and blood flow alterations over time in... Abstract
Cited 2 times since 2022 (0.9 per year) source: EuropePMC
Molecular therapy. Nucleic acids, Volume 28, 19 3 2022, Pages 307-327 Extracellular vesicles enriched with an endothelial cell pro-survival microRNA affects skin tissue regeneration. Fernandes H, Zonnari A, Abreu R, Aday S, Barão M, Albino I, Lino M, Branco A, Seabra C, Barata T, Leal EC, Tralhão JG, Gonçalves L, de Jong A, Peters HAB, de Vries MR, da Costa Martins P, Quax PHA, Ferreira L
Endothelial cell (EC) activity is essential for tissue regeneration in several (patho)physiological contexts. However, our capacity to deliver in vivo biomolecules capable of controlling EC fate is relatively limited. Here, we screened a library of microRNA (miR) mimics and identified 25 miRs capable of enhancing the survival of ECs exposed to ischemia-mimicking conditions. In vitro, we showed that miR-425-5p, one of the hits, was able to enhance EC survival and migration. In vivo, using a mouse... Abstract
Cited 11 times since 2022 (4.9 per year) source: EuropePMC
Frontiers in cardiovascular medicine, Volume 9, 14 2 2022, Pages 820962 The Intriguing Role of TLR Accessory Molecules in Cardiovascular Health and Disease. Bezhaeva T, Karper J, Quax PHA, de Vries MR
Activation of Toll like receptors (TLR) plays an important role in cardiovascular disease development, progression and outcomes. Complex TLR mediated signaling affects vascular and cardiac function including tissue remodeling and repair. Being central components of both innate and adaptive arms of the immune system, TLRs interact as pattern recognition receptors with a series of exogenous ligands and endogenous molecules or so-called danger associated molecular patterns (DAMPs) that are released... Abstract
Cited 6 times since 2022 (2.6 per year) source: EuropePMC
Frontiers in cardiovascular medicine, Volume 8, 3 1 2022, Pages 784980 Interfering in the ALK1 Pathway Results in Macrophage-Driven Outward Remodeling of Murine Vein Grafts. de Jong A, Sier VQ, Peters HAB, Schilder NKM, Jukema JW, Goumans MJTH, Quax PHA, de Vries MR
Aims: Vein grafts are frequently used to bypass coronary artery occlusions. Unfortunately, vein graft disease (VGD) causes impaired patency rates. ALK1 mediates signaling by TGF-β via TGFβR2 or BMP9/10 via BMPR2, which is an important pathway in fibrotic, inflammatory, and angiogenic processes in vascular diseases. The role of the TGF-β pathway in VGD is previously reported, however, the contribution of ALK1 signaling is not known. Therefore, we investigated ALK1 signaling in VGD in a mouse mode... Abstract
Cited 4 times since 2021 (1.6 per year) source: EuropePMC
International journal of molecular sciences, Volume 22, Issue 23, 24 4 2021, Pages 12678 Cold-Inducible RNA-Binding Protein but Not Its Antisense lncRNA Is a Direct Negative Regulator of Angiogenesis In Vitro and In Vivo via Regulation of the 14q32 angiomiRs-microRNA-329-3p and microRNA-495-3p. Goossens EAC, Zhang L, de Vries MR, Jukema JW, Quax PHA, Nossent AY
Inhibition of the 14q32 microRNAs, miR-329-3p and miR-495-3p, improves post-ischemic neovascularization. Cold-inducible RNA-binding protein (CIRBP) facilitates maturation of these microRNAs. We hypothesized that CIRBP deficiency improves post-ischemic angiogenesis via downregulation of 14q32 microRNA expression. We investigated these regulatory mechanisms both in vitro and in vivo. We induced hindlimb ischemia in Cirp-/- and C57Bl/6-J mice, monitored blood flow recovery with laser Doppler perfus... Abstract
Cited 3 times since 2021 (1.1 per year) source: EuropePMC
Biomedicines, Volume 9, Issue 10, 9 2 2021, Pages 1417 Perfusion Patterns in Patients with Chronic Limb-Threatening Ischemia versus Control Patients Using Near-Infrared Fluorescence Imaging with Indocyanine Green. Van Den Hoven P, Goncalves LN, Quax PHA, Van Rijswijk CSP, Van Schaik J, Schepers A, Vahrmeijer AL, Hamming JF, Van Der Vorst JR
In assessing the severity of lower extremity arterial disease (LEAD), physicians rely on clinical judgements supported by conventional measurements of macrovascular blood flow. However, current diagnostic techniques provide no information about regional tissue perfusion and are of limited value in patients with chronic limb-threatening ischemia (CLTI). Near-infrared (NIR) fluorescence imaging using indocyanine green (ICG) has been used extensively in perfusion studies and is a possible modality... Abstract
Cited 8 times since 2021 (2.9 per year) source: EuropePMC
Angiogenesis, Volume 25, Issue 1, 25 4 2021, Pages 129-143 PFKFB3 gene deletion in endothelial cells inhibits intraplaque angiogenesis and lesion formation in a murine model of venous bypass grafting. Perrotta P, de Vries MR, Peeters B, Guns PJ, De Meyer GRY, Quax PHA, Martinet W
Vein grafting is a frequently used surgical intervention for cardiac revascularization. However, vein grafts display regions with intraplaque (IP) angiogenesis, which promotes atherogenesis and formation of unstable plaques. Graft neovessels are mainly composed of endothelial cells (ECs) that largely depend on glycolysis for migration and proliferation. In the present study, we aimed to investigate whether loss of the glycolytic flux enzyme phosphofructokinase-2/fructose-2,6-bisphosphatase 3 (PF... Abstract
Cited 4 times since 2021 (1.4 per year) source: EuropePMC
Journal of cellular and molecular medicine, Volume 25, Issue 16, 30 5 2021, Pages 7772-7782 Phosphorylcholine antibodies restrict infarct size and left ventricular remodelling by attenuating the unreperfused post-ischaemic inflammatory response. Pluijmert NJ, de Jong RCM, de Vries MR, Pettersson K, Atsma DE, Jukema JW, Quax PHA
Phosphorylcholine is a pro-inflammatory epitope exposed on apoptotic cells, and phosphorylcholine monoclonal immunoglobulin (Ig)G antibodies (PC-mAb) have anti-inflammatory properties. In this study, we hypothesize that PC-mAb treatment reduces adverse cardiac remodelling and infarct size (IS) following unreperfused transmural myocardial infarction (MI). Unreperfused MI was induced by permanent ligation of the left anterior descending (LAD) coronary artery in hypercholesterolaemic APOE*3-Leiden... Abstract
Cited 10 times since 2021 (3.3 per year) source: EuropePMC
International journal of molecular sciences, Volume 22, Issue 9, 30 5 2021, Pages 4804 Endoglin/CD105-Based Imaging of Cancer and Cardiovascular Diseases: A Systematic Review. Sier VQ, van der Vorst JR, Quax PHA, de Vries MR, Zonoobi E, Vahrmeijer AL, Dekkers IA, de Geus-Oei LF, Smits AM, Cai W, Sier CFM, Goumans MJTH, Hawinkels LJAC
Molecular imaging of pathologic lesions can improve efficient detection of cancer and cardiovascular diseases. A shared pathophysiological feature is angiogenesis, the formation of new blood vessels. Endoglin (CD105) is a coreceptor for ligands of the Transforming Growth Factor-β (TGF-β) family and is highly expressed on angiogenic endothelial cells. Therefore, endoglin-based imaging has been explored to visualize lesions of the aforementioned diseases. This systematic review highlights the prog... Abstract
Cited 15 times since 2021 (4.9 per year) source: EuropePMC
Frontiers in cardiovascular medicine, Volume 8, 28 4 2021, Pages 647785 Post-ischemic Myocardial Inflammatory Response: A Complex and Dynamic Process Susceptible to Immunomodulatory Therapies. Pluijmert NJ, Atsma DE, Quax PHA
Following acute occlusion of a coronary artery causing myocardial ischemia and implementing first-line treatment involving rapid reperfusion, a dynamic and balanced inflammatory response is initiated to repair and remove damaged cells. Paradoxically, restoration of myocardial blood flow exacerbates cell damage as a result of myocardial ischemia-reperfusion (MI-R) injury, which eventually provokes accelerated apoptosis. In the end, the infarct size still corresponds to the subsequent risk of deve... Abstract
Cited 11 times since 2021 (3.5 per year) source: EuropePMC
Cells, Volume 10, Issue 4, 26 4 2021, Pages 737 The Inflammatory Profile of CTEPH-Derived Endothelial Cells Is a Possible Driver of Disease Progression. Smolders VFED, Lodder K, Rodríguez C, Tura-Ceide O, Barberà JA, Jukema JW, Quax PHA, Goumans MJ, Kurakula K
Chronic thromboembolic pulmonary hypertension (CTEPH) is a form of pulmonary hypertension characterized by the presence of fibrotic intraluminal thrombi and causing obliteration of the pulmonary arteries. Although both endothelial cell (EC) dysfunction and inflammation are linked to CTEPH pathogenesis, regulation of the basal inflammatory response of ECs in CTEPH is not fully understood. Therefore, in the present study, we investigated the role of the nuclear factor (NF)-κB pro-inflammatory sign... Abstract
Cited 35 times since 2021 (11.1 per year) source: EuropePMC
Biomedicines, Volume 9, Issue 4, 24 4 2021, Pages 328 Endothelial Barrier Function and Leukocyte Transmigration in Atherosclerosis. Sluiter TJ, van Buul JD, Huveneers S, Quax PHA, de Vries MR
The vascular endothelium is a highly specialized barrier that controls passage of fluids and migration of cells from the lumen into the vessel wall. Endothelial cells assist leukocytes to extravasate and despite the variety in the specific mechanisms utilized by different leukocytes to cross different vascular beds, there is a general principle of capture, rolling, slow rolling, arrest, crawling, and ultimately diapedesis via a paracellular or transcellular route. In atherosclerosis, the barrier... Abstract
Cited 2 times since 2021 (0.6 per year) source: EuropePMC
International journal of molecular sciences, Volume 22, Issue 4, 18 3 2021, Pages 2010 BMP Receptor Inhibition Enhances Tissue Repair in Endoglin Heterozygous Mice. Bakker W, Dingenouts CKE, Lodder K, Wiesmeijer KC, de Jong A, Kurakula K, Mager HJ, Smits AM, de Vries MR, Quax PHA, Goumans MJTH
Hereditary hemorrhagic telangiectasia type 1 (HHT1) is a severe vascular disorder caused by mutations in the TGFβ/BMP co-receptor endoglin. Endoglin haploinsufficiency results in vascular malformations and impaired neoangiogenesis. Furthermore, HHT1 patients display an impaired immune response. To date it is not fully understood how endoglin haploinsufficient immune cells contribute to HHT1 pathology. Therefore, we investigated the immune response during tissue repair in Eng+/- mice, a model for... Abstract
Cited 11 times since 2021 (3.4 per year) source: EuropePMC
Angiogenesis, Volume 24, Issue 3, 7 1 2021, Pages 567-581 Atorvastatin pleiotropically decreases intraplaque angiogenesis and intraplaque haemorrhage by inhibiting ANGPT2 release and VE-Cadherin internalization. Baganha F, de Jong RCM, Peters EA, Voorham W, Jukema JW, Delibegovic M, de Vries MR, Quax PHA
Objective: Statins pleiotropically provide additional benefits in reducing atherosclerosis, but their effects on intraplaque angiogenesis (IPA) and hemorrhage (IPH) remain unclear. Therefore, we discriminated statin's lipid-lowering dependent and independent effects on IPA and IPH. Approach and results: ApoE3*Leiden mice are statin-responsive due to ApoE and LDLR presence, but also allow to titrate plasma cholesterol levels by diet. Therefore, ApoE3*Leiden mice were fed a high-cholesterol-i... Abstract
Cited 6 times since 2021 (1.8 per year) source: EuropePMC
Journal of internal medicine, Volume 290, Issue 1, 2 1 2021, Pages 141-156 Identification of IgG1 isotype phosphorylcholine antibodies for the treatment of inflammatory cardiovascular diseases. de Vries MR, Ewing MM, de Jong RCM, MacArthur MR, Karper JC, Peters EAB, Nordzell M, Karabina SAP, Sexton D, Dahlbom I, Bergman A, Mitchell JR, Frostegård J, Kuiper J, Ninio E, Jukema JW, Pettersson K, Quax PHA
Background: Phosphorylcholine (PC) is an important pro-inflammatory damage-associated molecular pattern. Previous data have shown that natural IgM anti-PC protects against cardiovascular disease. We aimed to develop a monoclonal PC IgG antibody with anti-inflammatory and anti-atherosclerotic properties. Methods: Using various techniques PC antibodies were validated and optimized. In vivo testing was performed in a femoral artery cuff model in ApoE3*Leiden mice. Safety studies are performed in ra... Abstract
Cited 3 times since 2021 (0.9 per year) source: EuropePMC
Frontiers in cardiovascular medicine, Volume 7, 15 3 2021, Pages 604821 P300/CBP Associated Factor (PCAF) Deficiency Enhances Diet-Induced Atherosclerosis in ApoE3<sup>*</sup>Leiden Mice via Systemic Inhibition of Regulatory T Cells. de Jong A, de Jong RCM, Peters EA, Arens R, Jukema JW, de Vries MR, Quax PHA
Background: Inflammatory stimuli induced by NF-kB drive atherosclerotic lesion formation. The epigenetic P300/CBP associated factor (PCAF) post-transcriptionally acetylates FoxP3, which is required for regulatory T-cell (Treg) differentiation and immune modulation. We hypothesize that PCAF deficiency affects atherosclerosis via regulation of regulatory Tregs. Method: ApoE3*Leiden (n = 13) and ApoE3*LeidenxPCAF-/- (n = 13) were fed a high-fat diet (HFD) containing 1.25% cholesterol. Systemic FoxP... Abstract